ZEB1 monoclonal antibody, clone CL0151
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RH-30 and Lane 2: CACO-2 cell lysates with ZEB1 monoclonal antibody, clone CL0151 (Cat # MAB15542).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with ZEB1 monoclonal antibody, clone CL0151 (Cat # MAB15542) shows moderate to strong nuclear immunoreactivity in neuronal and glial cells.
Immunofluorescence
Immunofluorescent staining of A-549 cells with ZEB1 monoclonal antibody, clone CL0151 (Cat # MAB15542) (Green) shows nuclear (without nucleoli). Microtubule probes are visualized in red (where available).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ZEB1.
Immunogen
Recombinant protein corresponding to human ZEB1.
Sequence
EAEKPESSVSSATGDGNLSPSQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSEPSSPEPGKVNIPAKNNDQPQSANANEPQDSTVNLQSPLKMTNSPVLPVGST
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RH-30 and Lane 2: CACO-2 cell lysates with ZEB1 monoclonal antibody, clone CL0151 (Cat # MAB15542).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with ZEB1 monoclonal antibody, clone CL0151 (Cat # MAB15542) shows moderate to strong nuclear immunoreactivity in neuronal and glial cells.Immunofluorescence
Immunofluorescent staining of A-549 cells with ZEB1 monoclonal antibody, clone CL0151 (Cat # MAB15542) (Green) shows nuclear (without nucleoli). Microtubule probes are visualized in red (where available). -
Gene Info — ZEB1
Entrez GeneID
6935Protein Accession#
P37275Gene Name
ZEB1
Gene Alias
AREB6, BZP, DELTA-EF1, MGC133261, NIL-2-A, NIL-2A, NIL2A, TCF8, ZEB, ZFHEP, ZFHX1A
Gene Description
zinc finger E-box binding homeobox 1
Gene Ontology
HyperlinkGene Summary
ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM
Other Designations
OTTHUMP00000019405|transcription factor 8 (represses interleukin 2 expression)|zinc finger homeodomain enhancer-binding protein
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Interactome
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Disease
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Publication Reference
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Next-generation sequencing reveals novel differentially regulated mRNAs, lncRNAs, miRNAs, sdRNAs and a piRNA in pancreatic cancer.
Muller S, Raulefs S, Bruns P, Afonso-Grunz F, Plotner A, Thermann R, Jager C, Schlitter AM, Kong B, Regel I, Roth WK, Rotter B, Hoffmeier K, Kahl G, Koch I, Theis FJ, Kleeff J, Winter P, Michalski CW.
Molecular Cancer 2015 Apr; 14:94.
Application：IHC-P, Human, Human pancreatic cancer.
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Grainyhead-like 2 (GRHL2) distribution reveals novel pathophysiological differences between human idiopathic pulmonary fibrosis and mouse models of pulmonary fibrosis.
Varma S, Mahavadi P, Sasikumar S, Cushing L, Hyland T, Rosser AE, Riccardi D, Lu J, Kalin TV, Kalinichenko VV, Guenther A, Ramirez MI, Pardo A, Selman M, Warburton D.
American Journal of Physiology. Lung Cellular and Molecular Physiology 2014 Mar; 306(5):L405.
Application：IF, IHC-P, Human, Human lung.
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Next-generation sequencing reveals novel differentially regulated mRNAs, lncRNAs, miRNAs, sdRNAs and a piRNA in pancreatic cancer.
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