p44/42 ERK1/2 (phospho T202/Y204) monoclonal antibody, clone 2E8-2F6-C12
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More Files
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Western Blot (Cell lysate)
Western Blot analysis of p44/42 ERK1/2 (phospho T202/Y204) from EGF-treated A549 cells and PDGF-treated NIH/3T3 cells.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of human p44/42 ERK1/2.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T202/T204 of human p44/42 ERK1/2.
Host
Mouse
Theoretical MW (kDa)
44, 42
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
ELISA (1:10000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.5% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of p44/42 ERK1/2 (phospho T202/Y204) from EGF-treated A549 cells and PDGF-treated NIH/3T3 cells.Enzyme-linked Immunoabsorbent Assay
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