Phosphotyrosine monoclonal antibody, clone 7B4-D9-H10
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human phosphotyrosine.
Immunogen
A synthetic peptide corresponding to human Phosphotyrosine .
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG2a
Recommend Usage
ELISA (1:10000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.5% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of extracts from A549 cells untreated and treated with epidermal growth factor (10 ng/mL, 15min). The phospho-specificity of the antibody was further characterized by treating the membrane with alkaline phosphatase after Western transfer.Enzyme-linked Immunoabsorbent Assay
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