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Last updated: 2017/5/21

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Phosphotyrosine monoclonal antibody, clone 7B4-D9-H10

  • Catalog # : MAB15283
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of human phosphotyrosine.
  • Immunogen:
  • A synthetic peptide corresponding to human Phosphotyrosine .
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • ELISA (1:10000-1:20000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 0.5% BSA, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of extracts from A549 cells untreated and treated with epidermal growth factor (10 ng/mL, 15min). The phospho-specificity of the antibody was further characterized by treating the membrane with alkaline phosphatase after Western transfer.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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  • ELISA
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