Histone H2AX (phospho S139) monoclonal antibody, clone 8H4-1H11-F8
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of phosphorylation of H2AX at Serine 139 in 3T3 or hydroxyurea-treated 3T3 cell lysates. Predicted band size: 15 KDa. Observed band size: 15 KDa.
Immunofluorescence
Immunofluorescence staining of phosphorylation of H2AX at Serine 139 in 3T3 or hydroxyurea-treated 3T3 cells.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of human histone H2AX.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S139 of human histone H2AX.
Host
Mouse
Theoretical MW (kDa)
15
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.5% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of phosphorylation of H2AX at Serine 139 in 3T3 or hydroxyurea-treated 3T3 cell lysates. Predicted band size: 15 KDa. Observed band size: 15 KDa.Immunofluorescence
Immunofluorescence staining of phosphorylation of H2AX at Serine 139 in 3T3 or hydroxyurea-treated 3T3 cells. -
Gene Info — H2AFX
Entrez GeneID
3014Protein Accession#
P16104Gene Name
H2AFX
Gene Alias
H2A.X, H2A/X, H2AX
Gene Description
H2A histone family, member X
Omim ID
601772Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
Other Designations
H2AX histone
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Interactome
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Pathway
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Disease
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