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Last updated: 2017/10/15

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SMAD3 monoclonal antibody, clone 5G11

  • Catalog # : MAB15274
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human SMAD3.
  • Immunogen:
  • Recombinant protein corresponding to human SMAD3.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • ELISA (1:10000)
    Immunofluorescence (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascitic fluid (0.03% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Line 1: A549, Line 2: HeLa, Line 3: Jurkat, Line 4: PC-2 and Line 5: NIH/3T3 cell lysate.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of NIH/3T3 cells using SMAD3 monoclonal antibody, clone 5G11 (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4088
  • Gene Name:
  • SMAD3
  • Gene Alias:
  • DKFZp586N0721,DKFZp686J10186,HSPC193,HsT17436,JV15-2,MADH3,MGC60396
  • Gene Description:
  • SMAD family member 3
  • Gene Summary:
  • The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq
  • Other Designations:
  • MAD, mothers against decapentaplegic homolog 3,SMA- and MAD-related protein 3,SMAD, mothers against DPP homolog 3,mad homolog JV15-2,mad protein homolog,mothers against decapentaplegic homolog 3
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