RUNX1 monoclonal antibody, clone 5A1
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell.
Immunofluorescence
Immunofluorescence staining of HeLa cells using RUNX1 monoclonal antibody, clone 5A1 (Green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RUNX1 monoclonal antibody, clone 5A1.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human RUNX1.
Immunogen
A synthetic peptide corresponding to C-terminus of human RUNX1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Recommend Usage
ELISA (1:10000)
Immunofluorescence (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic fluid (0.03% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell.Immunofluorescence
Immunofluorescence staining of HeLa cells using RUNX1 monoclonal antibody, clone 5A1 (Green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RUNX1 monoclonal antibody, clone 5A1. -
Gene Info — RUNX1
Entrez GeneID
861Protein Accession#
Q01196Gene Name
RUNX1
Gene Alias
AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB
Gene Description
runt-related transcription factor 1
Gene Ontology
HyperlinkGene Summary
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
AML1-EVI-1 fusion protein|acute myeloid leukemia 1|aml1 oncogene|core-binding factor, runt domain, alpha subunit 2
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Interactome
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Pathway
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Disease
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