GABPA monoclonal antibody, clone 8C1B10
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Line 1: HeLa, Line 2: A549, Line 3: MCF-7, Line 4: NIH/3T3 and Line 5: SMMC-7721 cell lysate.
Immunofluorescence
Immunofluorescence staining of HeLa cells using GABPA monoclonal antibody, clone 8C1B10 (Green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human GABPA.
Immunogen
Recombinant protein corresponding to amino acids 120-190 of human GABPA.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Recommend Usage
ELISA (1:10000)
Immunofluorescence (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic fluid (0.03% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Line 1: HeLa, Line 2: A549, Line 3: MCF-7, Line 4: NIH/3T3 and Line 5: SMMC-7721 cell lysate.Immunofluorescence
Immunofluorescence staining of HeLa cells using GABPA monoclonal antibody, clone 8C1B10 (Green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GABPA
Entrez GeneID
2551Protein Accession#
Q06546Gene Name
GABPA
Gene Alias
E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A
Gene Description
GA binding protein transcription factor, alpha subunit 60kDa
Omim ID
600609Gene Ontology
HyperlinkGene Summary
This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. [provided by RefSeq
Other Designations
GA binding protein transcription factor, alpha subunit|OTTHUMP00000096114|human nuclear respiratory factor-2 subunit alpha|nuclear respiratory factor 2 alpha subunit
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Interactome
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Disease
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