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CHEK2 monoclonal antibody, clone 1C12B8

  • Catalog # : MAB15266
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human CHEK2.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 481-531 of human CHEK2.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • ELISA (1:10000)
    Immunofluorescence (1:200-1:1000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascitic fluid (0.03% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Line 1: HeLa, Line 2: ME-180, Line3: COS, Line 4: 293, Line 5: HCT-116, Line 6: SH-SY5Y, Line 7: HT-29 and Line 8: MCF-7 cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human lung carcinoma (B) human liver carcinoma (C) human breast carcinoma and (D) human kiney carcinoma, showing nuclear localization with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of HeLa cells using CHEK2 monoclonal antibody, clone 1C12B8 (Green), showing nuclear localization. Red: Actin filaments have been labeled with DY-554 phalloidin.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • CHEK2
  • Gene Alias:
  • CDS1,CHK2,HuCds1,LFS2,PP1425,RAD53
  • Gene Description:
  • CHK2 checkpoint homolog (S. pombe)
  • Gene Summary:
  • In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000028871,checkpoint-like protein CHK2,protein kinase CHK2,serine/threonine-protein kinase CHK2
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