CD14 monoclonal antibody, clone 5A3
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CD14.
Immunogen
Recombinant protein corresponding to 30-60 at the N-terminus of human CD14.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.03% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymph node, showing membrane localization with DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of human PBMC. -
Gene Info — CD14
Entrez GeneID
929Protein Accession#
P08571Gene Name
CD14
Gene Alias
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Gene Description
CD14 molecule
Omim ID
158120Gene Ontology
HyperlinkGene Summary
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq
Other Designations
CD14 antigen|monocyte differentiation antigen CD14
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Interactome
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Pathway
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Disease
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