JUP monoclonal antibody, clone 4C12
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human JUP.
Immunogen
Recombinant protein corresponding to human JUP.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Recommend Usage
ELISA (1:10000)
Immunofluorescence (1:200-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic fluid (0.03% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Line 1: T47D, Line 2: MCF-7, Line 3: SKBR-3, Line 4: A431 and Line 5: HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rectum.Immunofluorescence
Immunofluorescence staining of U251 cells using JUP monoclonal antibody, clone 4C12 (Green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of JUP monoclonal antibody, clone 4C12. -
Gene Info — JUP
Entrez GeneID
3728Protein Accession#
P14923Gene Name
JUP
Gene Alias
ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB
Gene Description
junction plakoglobin
Gene Ontology
HyperlinkGene Summary
This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq
Other Designations
OTTHUMP00000164735|catenin (cadherin-associated protein), gamma (80kD)|catenin (cadherin-associated protein), gamma 80kDa|gamma-catenin
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Interactome
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Disease
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