BCL10 monoclonal antibody, clone 4F8
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH/3T3, Lane 2: HeLa, Lane 3: MCF-7 and Lane 4: Jurkat cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human breast carcinoma and (B) liver carcinoma, showing cytoplasmic localization with DAB staining.
Immunofluorescence
Immunofluorescence staining of HeLa cells using BCL10 monoclonal antibody, clone 4F8 (Green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of HeLa cells using BCL10 monoclonal antibody, clone 4F8 (Green) and negative control (Purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human BCL10.
Immunogen
Recombinant protein corresponding to human BCL10.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.03% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH/3T3, Lane 2: HeLa, Lane 3: MCF-7 and Lane 4: Jurkat cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human breast carcinoma and (B) liver carcinoma, showing cytoplasmic localization with DAB staining.Immunofluorescence
Immunofluorescence staining of HeLa cells using BCL10 monoclonal antibody, clone 4F8 (Green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using BCL10 monoclonal antibody, clone 4F8 (Green) and negative control (Purple). -
Gene Info — BCL10
Entrez GeneID
8915Protein Accession#
O95999Gene Name
BCL10
Gene Alias
CARMEN, CIPER, CLAP, c-E10, mE10
Gene Description
B-cell CLL/lymphoma 10
Omim ID
603517Gene Ontology
HyperlinkOther Designations
CARD containing molecule enhancing NF-kB|CARD-containing apoptotic signaling protein|CARD-containing proapoptotic protein|CARD-like apoptotic protein|OTTHUMP00000011647|OTTHUMP00000036080|caspase-recruiting domain-containing protein
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Interactome
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Pathway
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Disease
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