PRDX1 monoclonal antibody, clone 8e7
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: MCF7 cell, Lane 2: rat kidney tissue and Lane 3: mouse brain tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human lung tissue. MAB15254 at 1:200 (4°C, overnight). Antigen retrieval - Sodium Citrate pH6 (>98°C, 20 min). Secondary - 1:200 (room temp., 30 min). Negative control - Secondary only.
Immunofluorescence
Immunofluorescence staining of HeLa cell line with antibody (Left), DAPI (Middle) and merge (Right).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human PRDX1.
Immunogen
Recombinant protein corresponding to human PRDX1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype
IgG1
Recommend Usage
Immunofluorescence (1:100-1:200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:300)
Western Blot (1:1000-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.5% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: MCF7 cell, Lane 2: rat kidney tissue and Lane 3: mouse brain tissue.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human lung tissue. MAB15254 at 1:200 (4°C, overnight). Antigen retrieval - Sodium Citrate pH6 (>98°C, 20 min). Secondary - 1:200 (room temp., 30 min). Negative control - Secondary only.Immunofluorescence
Immunofluorescence staining of HeLa cell line with antibody (Left), DAPI (Middle) and merge (Right). -
Gene Info — PRDX1
Entrez GeneID
5052Protein Accession#
Q06830Gene Name
PRDX1
Gene Alias
MSP23, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2
Gene Description
peroxiredoxin 1
Omim ID
176763Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000009234|natural killer-enhancing factor A|proliferation-associated gene A|thioredoxin-dependent peroxide reductase 2
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Interactome
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Disease
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