His tag monoclonal antibody, clone 4E6
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More Files
- More Functions
Western Blot (Recombinant protein)
Western Blot analysis of 2 ug His fusion protein. Lane 1: At 1:5000 dilution, Lane 2: At 1:10000 dilution.
Immunofluorescence
Immunofluorescence staining of 293 cells transfected with a His-tag protein using antibody (Red) and DAPI (Blue).
Immunoprecipitation
Immunoprecipitation analysis of Lane 1: Untransfected 293 cell lysate, Lane 2: Transfected 293 cell lysate with His-tag fusion protein, Lane 3: IP (untransfected 293 + His tag monoclonal antibody, clone 4E6 + Protein G agarose) , Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose), Lane 5: IP (transfected 293 + His tag monoclonal antibody, clone 4E6 + Protein G agarose), Lane 6: Recombinant protein (E.coli).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human His tag.
Immunogen
A synthetic peptide corresponding to full length human His tag.
Host
Mouse
Specificity
The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.
Form
Liquid
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1:1000)
Immunoprecipitation (1:200)
Western Blot (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.5% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western Blot analysis of 2 ug His fusion protein. Lane 1: At 1:5000 dilution, Lane 2: At 1:10000 dilution.Immunofluorescence
Immunofluorescence staining of 293 cells transfected with a His-tag protein using antibody (Red) and DAPI (Blue).Immunoprecipitation
Immunoprecipitation analysis of Lane 1: Untransfected 293 cell lysate, Lane 2: Transfected 293 cell lysate with His-tag fusion protein, Lane 3: IP (untransfected 293 + His tag monoclonal antibody, clone 4E6 + Protein G agarose) , Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose), Lane 5: IP (transfected 293 + His tag monoclonal antibody, clone 4E6 + Protein G agarose), Lane 6: Recombinant protein (E.coli).
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