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His tag monoclonal antibody, clone 4E6

  • Catalog # : MAB15243
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of human His tag.
  • Immunogen:
  • A synthetic peptide corresponding to full length of human His tag.
  • Host:
  • Mouse
  • Specificity:
  • The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunofluorescence (1:1000)
    Immunoprecipitation (1:200)
    Western Blot (1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 0.5% BSA, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western Blot analysis of 2 ug His fusion protein. Lane 1: At 1:5000 dilution, Lane 2: At 1:10000 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of 293 cells transfected with a His-tag protein using antibody (Red) and DAPI (Blue).
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation analysis of Lane 1: Untransfected 293 cell lysate, Lane 2: Transfected 293 cell lysate with His-tag fusion protein, Lane 3: IP (untransfected 293 + His tag monoclonal antibody, clone 4E6 + Protein G agarose) , Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose), Lane 5: IP (transfected 293 + His tag monoclonal antibody, clone 4E6 + Protein G agarose), Lane 6: Recombinant protein (E.coli).
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
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