KRT5/KRT8 monoclonal antibody, clone C-50
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Specification
Product Description
Mouse monoclonal antibody raised against native human KRT5 and KRT8.
Immunogen
Cytoskeletal preparation from HeLa cells.
Host
Mouse
Theoretical MW (kDa)
58, 52.5
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Western Blotting (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with KRT5/KRT8 monoclonal antibody, clone C-50 (Cat # MAB15238).Immunofluorescence
Flow Cytometry
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Gene Info — KRT5
Entrez GeneID
3852Protein Accession#
P13647;P05787Gene Name
KRT5
Gene Alias
CK5, DDD, EBS2, K5, KRT5A
Gene Description
keratin 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq
Other Designations
58 kda cytokeratin|epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types|keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)|keratin, type II cytoskeletal 5
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Gene Info — KRT8
Entrez GeneID
3856Protein Accession#
P13647;P05787Gene Name
KRT8
Gene Alias
CARD2, CK8, CYK8, K2C8, K8, KO
Gene Description
keratin 8
Gene Ontology
HyperlinkGene Summary
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq
Other Designations
cytokeratin 8|keratin, type II cytoskeletal 8
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Interactome
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Disease
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Publication Reference
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A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.
Bártek J, Vojtĕsek B, Stasková Z, Bártková J, Kerekés Z, Rejthar A, Kovarík J.
The Journal of Pathology 1991 Jul; 164(3):215.
Application:IF, WB-Ce, Human, MCF-7, PMC42, A431, HeLa cells.
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Effects of tissue fixation conditions and protease pretreatment on immunohistochemical performance of a large series of new anti-keratin monoclonal antibodies: value in oncopathology.
Bártková J, Bártek J, Lukás Z, Vojtĕsek B, Stasková Z, Bursová H, Pavlovská R, Rejthar A, Kovarík J.
Neoplasma 1991 Feb; 38(4):439.
Application:IHC-P, Human, Protease.
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A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.
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