Nefh monoclonal antibody, clone N52
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Specification
Product Description
Mouse monoclonal antibody raised against native Nefh.
Immunogen
Native purified pig neurofilament 200.
Host
Mouse
Theoretical MW (kDa)
200
Reactivity
Human, Mouse, Pig, Rat
Form
Lyophilized
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
Immunohistochemistry (Frozen sections) (1-2 ug/mL)
Western Blot (0.5 mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from ascites fluid, 1.2% sodium acetate (2 mg BSA, 0.01 mg sodium azide)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 1 mL of PBS buffer and concentration will be 100 ug/mL, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of rat brain tissue.Immunohistochemistry (Frozen sections)
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Publication Reference
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Charcot-Marie-Tooth disease neurofilament mutations disrupt neurofilament assembly and axonal transport.
Brownlees J, Ackerley S, Grierson AJ, Jacobsen NJ, Shea K, Anderton BH, Leigh PN, Shaw CE, Miller CC.
Human Molecular Genetics 2002 Nov; 11(23):2837.
Application:WB-Tr, Human, SW13 cells.
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The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it.
Lees JF, Shneidman PS, Skuntz SF, Carden MJ, Lazzarini RA.
The EMBO Journal 1988 Jul; 7(7):1947.
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Charcot-Marie-Tooth disease neurofilament mutations disrupt neurofilament assembly and axonal transport.
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