Pan Cytokeratin monoclonal antibody, clone SPM115 + SPM116
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against native human Pan Cytokeratin.
Immunogen
Human epidermal keratin.
Host
Mouse
Theoretical MW (kDa)
40-67
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
Western Blotting (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma with Pan Cytokeratin monoclonal antibody, clone SPM115 + SPM116 (Cat # MAB15226).Immunofluorescence
Flow Cytometry
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Gene Info — KRT1
Entrez GeneID
3848Protein Accession#
P04264;P12035;P19013;P13647;P02538;P04259;P48668;P05787;P13645;P02533;P19012;P08779;P08727Gene Name
KRT1
Gene Alias
CK1, EHK1, K1, KRT1A
Gene Description
keratin 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq
Other Designations
cytokeratin 1|epidermolytic hyperkeratosis 1|hair alpha protein|keratin, type II cytoskeletal 1
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Gene Info — KRT3
Entrez GeneID
3850Protein Accession#
P04264;P12035;P19013;P13647;P02538;P04259;P48668;P05787;P13645;P02533;P19012;P08779;P08727Gene Name
KRT3
Gene Alias
CK3, FLJ95909, K3
Gene Description
keratin 3
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq
Other Designations
65 kDa cytokeratin|cytokeratin 3|keratin, type II cytoskeletal 3
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Gene Info — KRT4
Entrez GeneID
3851Protein Accession#
P04264;P12035;P19013;P13647;P02538;P04259;P48668;P05787;P13645;P02533;P19012;P08779;P08727Gene Name
KRT4
Gene Alias
CK4, CYK4, FLJ31692, K4
Gene Description
keratin 4
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq
Other Designations
cytokeratin 4|keratin, type II cytoskeletal 4
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Gene Info — KRT5
Entrez GeneID
3852Protein Accession#
P04264;P12035;P19013;P13647;P02538;P04259;P48668;P05787;P13645;P02533;P19012;P08779;P08727Gene Name
KRT5
Gene Alias
CK5, DDD, EBS2, K5, KRT5A
Gene Description
keratin 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq
Other Designations
58 kda cytokeratin|epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types|keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)|keratin, type II cytoskeletal 5
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Gene Info — KRT6A
Entrez GeneID
3853Protein Accession#
P04264;P12035;P19013;P13647;P02538;P04259;P48668;P05787;P13645;P02533;P19012;P08779;P08727Gene Name
KRT6A
Gene Alias
CK6A, CK6C, CK6D, K6A, K6C, K6D, KRT6C, KRT6D
Gene Description
keratin 6A
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq
Other Designations
56 cytoskeletal type II keratin|K6D keratin|cytokeratin 6A|cytokeratin 6C|cytokeratin 6D|keratin 6C|keratin, epidermal type II, K6A|keratin, epidermal type II, K6C|keratin, type II cytoskeletal 6D|type II keratin isoform K6c
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Gene Info — KRT6B
Entrez GeneID
3854Protein Accession#
P04264;P12035;P19013;P13647;P02538;P04259;P48668;P05787;P13645;P02533;P19012;P08779;P08727Gene Name
KRT6B
Gene Alias
CK6B, K6B, KRTL1, PC2
Gene Description
keratin 6B
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq
Other Designations
cytokeratin 6B|keratin, epidermal, type II, K6B|keratin, type II cytoskeletal 6B|keratin-like 1 (a type II keratin sequence)
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Gene Info — KRT8
Entrez GeneID
3856Protein Accession#
P04264;P12035;P19013;P13647;P02538;P04259;P48668;P05787;P13645;P02533;P19012;P08779;P08727Gene Name
KRT8
Gene Alias
CARD2, CK8, CYK8, K2C8, K8, KO
Gene Description
keratin 8
Gene Ontology
HyperlinkGene Summary
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq
Other Designations
cytokeratin 8|keratin, type II cytoskeletal 8
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Gene Info — KRT10
Entrez GeneID
3858Protein Accession#
P04264;P12035;P19013;P13647;P02538;P04259;P48668;P05787;P13645;P02533;P19012;P08779;P08727Gene Name
KRT10
Gene Alias
CK10, K10, KPP
Gene Description
keratin 10
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq
Other Designations
cytokeratin 10
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Gene Info — KRT6C
Entrez GeneID
286887Protein Accession#
P04264;P12035;P19013;P13647;P02538;P04259;P48668;P05787;P13645;P02533;P19012;P08779;P08727Gene Name
KRT6C
Gene Alias
K6E, KRT6E, MGC102925, MGC163455, MGC163457
Gene Description
keratin 6C
Gene Ontology
HyperlinkGene Summary
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq
Other Designations
keratin 6E
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Interactome
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Disease
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Publication Reference
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Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
J Woodcock-Mitchell, R Eichner, W G Nelson, T T Sun.
The Journal of Cell Biology 1982 Nov; 95(2 Pt 1):580.
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Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies.
S C Tseng, M J Jarvinen, W G Nelson, J W Huang, J Woodcock-Mitchell, T T Sun.
Cell 1982 Sep; 30(2):361.
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Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
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