TYMS monoclonal antibody, clone SPM453
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Specifications
Product Description
Mouse monoclonal antibody raised against full length recombinant human TYMS.
Immunogen
Recombinant protein corresponding to full length human TYMS.
Host
Mouse
Theoretical MW (kDa)
36
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testicle tumor with TYMS monoclonal antibody, clone SPM453 (Cat # MAB15219).Immunofluorescence
Flow Cytometry
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Gene Info — TYMS
Entrez GeneID
7298Protein Accession#
P04818Gene Name
TYMS
Gene Alias
HsT422, MGC88736, TMS, TS, TSase
Gene Description
thymidylate synthetase
Omim ID
188350Gene Ontology
HyperlinkGene Summary
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq
Other Designations
Thymidylate synthase
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Immunological quantitation of thymidylate synthase using the monoclonal antibody TS 106 in 5-fluorouracil-sensitive and -resistant human cancer cell lines.
Johnston PG, Drake JC, Trepel J, Allegra CJ.
Cancer Research 1992 Aug; 52(16):4306.
Application:Flow Cyt, IF, WB-Ce, Human, AGS, HCT-116, MCF-7, NCI H630, SNU cells.
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Production and characterization of monoclonal antibodies that localize human thymidylate synthase in the cytoplasm of human cells and tissue.
Johnston PG, Liang CM, Henry S, Chabner BA, Allegra CJ.
Cancer Research 1991 Dec; 51(24):6668.
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Immunological quantitation of thymidylate synthase using the monoclonal antibody TS 106 in 5-fluorouracil-sensitive and -resistant human cancer cell lines.
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