TNF monoclonal antibody, clone SPM543
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human TNF.
Immunogen
A hexadecapeptide (conjugated with thyroglobulin) corresponding to amino acids 115-130 of human TNF.
Host
Mouse
Theoretical MW (kDa)
17
Reactivity
Human
Form
Liquid
Isotype
IgM, kappa
Recommend Usage
Flow Cytometry (1-2 ug/million cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human erdheim chester disease (also known as polyostotic sclerosing histiocytosis) with TNF monoclonal antibody, clone SPM543 (Cat # MAB15217).Immunofluorescence
Flow Cytometry
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Gene Info — TNF
Entrez GeneID
7124Protein Accession#
P01375Gene Name
TNF
Gene Alias
DIF, TNF-alpha, TNFA, TNFSF2
Gene Description
tumor necrosis factor (TNF superfamily, member 2)
Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq
Other Designations
APC1 protein|OTTHUMP00000029281|OTTHUMP00000037669|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry.
Z Bebok, B Markus, P Nemeth.
Breast Cancer Research and Treatment 1994 Jan; 29(3):229.
Application:IHC-Fr, IHC-P, Human, Human breast cancer.
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Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry.
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