IVL monoclonal antibody, clone SPM259
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Specification
Product Description
Mouse monoclonal antibody raised against native human IVL.
Immunogen
Native purified IVL from human keratinocytes.
Host
Mouse
Theoretical MW (kDa)
66-170
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-0.2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with IVL monoclonal antibody, clone SPM259 (Cat # MAB15198).Immunofluorescence
Flow Cytometry
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Gene Info — IVL
Entrez GeneID
3713Protein Accession#
P07476Gene Name
IVL
Gene Alias
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Gene Description
involucrin
Omim ID
147360Gene Ontology
HyperlinkGene Summary
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq
Other Designations
OTTHUMP00000014402
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Interactome
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Disease
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Publication Reference
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Characterisation of eight monoclonal antibodies to involucrin.
Hudson DL, Weiland KL, Dooley TP, Simon M, Watt FM.
Hybridoma 1992 Jun; 11(3):367.
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Characterisation of eight monoclonal antibodies to involucrin.
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