Phosphotyrosine monoclonal antibody, clone SPM102
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma with Phosphotyrosine monoclonal antibody, clone SPM102 (Cat # MAB15197).
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Specification
Product Description
Mouse monoclonal antibody raised against native phosphotyrosine.
Immunogen
Phosphotyrosine conjugated to KLH.
Host
Mouse
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2b
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
Immunoprecipitation (1-2 ug/500 ug protein lysate)
Western Blotting (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma with Phosphotyrosine monoclonal antibody, clone SPM102 (Cat # MAB15197).Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Publication Reference
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Monoclonal antibodies to phosphotyrosine.
Glenney JR Jr, Zokas L, Kamps MP.
Journal of Immunological Methods 1988 May; 109(2):277.
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Monoclonal antibodies to phosphotyrosine.
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