Mouse monoclonal antibody raised against native phosphotyrosine.
Phosphotyrosine conjugated to KLH.
Protein A/G purification
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL) Immunofluorescence (1-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL) Immunoprecipitation (1-2 ug/500 ug protein lysate) Western Blotting (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
In 10 mM PBS.
Store at -20 to -80°C. Aliquot to avoid repeated freezing and thawing.