AKT2 monoclonal antibody, clone 1B6
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) A431 , (2) Jurkat, (3) HEK293, (4) A549, (5) MCF-7 and (6) PC-12 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human thymus tissues with AEC.
Immunofluorescence
Immunofluorescence staining of PANC-1 (left) and HeLa (right) cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human AKT2.
Immunogen
Recombinant protein corresponding to human AKT2.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Immunofluorescence (1:200-1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic fluid (0.03% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) A431 , (2) Jurkat, (3) HEK293, (4) A549, (5) MCF-7 and (6) PC-12 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human thymus tissues with AEC.Immunofluorescence
Immunofluorescence staining of PANC-1 (left) and HeLa (right) cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT2
Entrez GeneID
208Gene Name
AKT2
Gene Alias
PKBB, PKBBETA, PRKBB, RAC-BETA
Gene Description
v-akt murine thymoma viral oncogene homolog 2
Gene Ontology
HyperlinkGene Summary
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
Other Designations
Murine thymoma viral (v-akt) homolog-2|rac protein kinase beta
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Interactome
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Pathway
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Disease
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