CD63 monoclonal antibody, clone SPM524
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Specification
Product Description
Mouse monoclonal antibody raised against human CD63.
Immunogen
Smooth plasma membrane fraction of MeWo cells
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at 4°C. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western Blot (Tissue lysate) analysis of human spleen.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human melanoma and (B) mouse spleen.Immunofluorescence
Flow Cytometry
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Gene Info — CD63
Entrez GeneID
967Protein Accession#
P08962Gene Name
CD63
Gene Alias
LAMP-3, ME491, MLA1, OMA81H, TSPAN30
Gene Description
CD63 molecule
Omim ID
155740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq
Other Designations
CD63 antigen|CD63 antigen (melanoma 1 antigen)|granulophysin|lysosome-associated membrane glycoprotein 3|melanoma 1 antigen|melanoma-associated antigen ME491|melanoma-associated antigen MLA1|ocular melanoma-associated antigen|tetraspanin-30
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Interactome
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Pathway
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Publication Reference
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Extracellular vesicles from human liver stem cells inhibit tumor angiogenesis.
Lopatina T, Grange C, Fonsato V, Tapparo M, Brossa A, Fallo S, Pitino A, Herrera-Sanchez MB, Kholia S, Camussi G, Bussolati B.
International Journal of Cancer 2018 Aug; [Epub].
Application:Flow Cyt, Human, HLSC-Evs, MSC-EVs.
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Correlation of histopathological characteristics with staining patterns in human melanoma assessed by (monoclonal) antibodies reactive on paraffin sections.
Hagen EC, Vennegoor C, Schlingemann RO, van der Velde EA, Ruiter DJ.
Histopathology 1986 Jul; 10(7):689.
Application:IHC-P, Human, Human melanomas.
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A comparison of some methods for identifying amelanotic and oligomelanotic melanoma metastases in paraffin sections.
Palmer AA, Hall BE, Lew M.
Pathology 1985 Apr; 17(2):335.
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Biochemical characterization and cellular localization of a formalin-resistant melanoma-associated antigen reacting with monoclonal antibody NKI/C-3.
Vennegoor C, Calafat J, Hageman P, van Buitenen F, Janssen H, Kolk A, Rümke P.
International Journal of Cancer 1985 Mar; 35(3):287.
Application:Func, IHC-Fr, IHC-P, Human , Human melanomas.
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Extracellular vesicles from human liver stem cells inhibit tumor angiogenesis.
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