AR monoclonal antibody, clone RM254
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 22RV1 whole cell lysates with AR monoclonal antibody, clone RM254 (Cat # MAB15132), showed full length AR and splice variants expressed in 22RV1 cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human prostate cancer and (B) 22RV1 cells with AR monoclonal antibody, clone RM254 (Cat # MAB15132) at a 1:2500 dilution.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human AR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to N-terminus of human AR.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to full length androgen receptor and splice variants.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:2500)
Western Blot (1:100-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 22RV1 whole cell lysates with AR monoclonal antibody, clone RM254 (Cat # MAB15132), showed full length AR and splice variants expressed in 22RV1 cells.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human prostate cancer and (B) 22RV1 cells with AR monoclonal antibody, clone RM254 (Cat # MAB15132) at a 1:2500 dilution. -
Gene Info — AR
Entrez GeneID
367Protein Accession#
P10275Gene Name
AR
Gene Alias
AIS, DHTR, HUMARA, KD, NR3C4, SBMA, SMAX1, TFM
Gene Description
androgen receptor
Gene Ontology
HyperlinkGene Summary
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000023450|OTTHUMP00000061928|dihydrotestosterone receptor
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Interactome
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Pathway
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Disease
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