MCAM monoclonal antibody, clone RM249
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A375 cell lysates with MCAM monoclonal antibody, clone RM249 (Cat # MAB15130) at a 1:1000 dilution, showed MCAM (~110 kDa) expression in A375 cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with MCAM monoclonal antibody, clone RM249 (Cat # MAB15130) at a 1:400 dilution.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human MCAM.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to C-terminus of human MCAM.
Sequence
N/A
Reactivity
Human
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A375 cell lysates with MCAM monoclonal antibody, clone RM249 (Cat # MAB15130) at a 1:1000 dilution, showed MCAM (~110 kDa) expression in A375 cells.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with MCAM monoclonal antibody, clone RM249 (Cat # MAB15130) at a 1:400 dilution. -
Gene Info — MCAM
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Interactome
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