GFAP monoclonal antibody, clone RM246
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysates with GFAP monoclonal antibody, clone RM246 (Cat # MAB15128) at a 1:2500 dilution, showed GFAP (~50 kDa) expression in mouse brain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain with GFAP monoclonal antibody, clone RM246 (Cat # MAB15128) at 1:500 dilution.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human GFAP.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to N-terminus of human GFAP.
Sequence
N/A
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:1000-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysates with GFAP monoclonal antibody, clone RM246 (Cat # MAB15128) at a 1:2500 dilution, showed GFAP (~50 kDa) expression in mouse brain.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain with GFAP monoclonal antibody, clone RM246 (Cat # MAB15128) at 1:500 dilution. -
Gene Info — GFAP
Entrez GeneID
2670Protein Accession#
P14136Gene Name
GFAP
Gene Alias
FLJ45472
Gene Description
glial fibrillary acidic protein
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
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Interactome
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Disease
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