HIF1A monoclonal antibody, clone RM242
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cells, Lane 2: Jurkat cells treated with Cobalt(II) chloride (CoCl2) using HIF1A monoclonal antibody, clone RM242 (Cat # MAB15126) at a 1:1000 dilution, showed that HIF1A (~120 kDa) expression was induced by CoCl2 in Jurkat cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with HIF1A monoclonal antibody, clone RM242 (Cat # MAB15126) at 1:1000 dilution.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human HIF1A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to internal region of human HIF1A.
Sequence
N/A
Reactivity
Human
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cells, Lane 2: Jurkat cells treated with Cobalt(II) chloride (CoCl2) using HIF1A monoclonal antibody, clone RM242 (Cat # MAB15126) at a 1:1000 dilution, showed that HIF1A (~120 kDa) expression was induced by CoCl2 in Jurkat cells.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with HIF1A monoclonal antibody, clone RM242 (Cat # MAB15126) at 1:1000 dilution. -
Gene Info — HIF1A
Entrez GeneID
3091Protein Accession#
Q16665Gene Name
HIF1A
Gene Alias
HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Gene Description
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Omim ID
603348Gene Ontology
HyperlinkGene Summary
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
ARNT interacting protein|hypoxia-inducible factor 1, alpha subunit|hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|member of PAS superfamily 1
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Interactome
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Pathway
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Disease
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