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Histone H2B monoclonal antibody, clone RM230

  • Catalog # : MAB15119
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human histone H2B.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human histone H2B.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.2-1 ug/mL)
    Immunocytochemistry (0.5-1 ug/mL)
    Multiplex (0.1-1 ug/mL)
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: recombinant Histone H2A, Lane 2: recombinant Histone H2B, Lane 3: HeLa, Lane 4: A375, Lane 5: SK-MEL-2, Lane 6: A431, Lane 7: K562 whole cell lysates with Histone H2B monoclonal antibody, clone RM230 (Cat # MAB15119) at 0.2 ug/mL working concentration.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of HeLa cells with Histone H2B monoclonal antibody, clone RM230 (Cat # MAB15119) (Red). Actin filaments was labeled with fluorescein phalloidin (Green).
  • ELISA
  • Application Image
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 8349
  • Gene Name:
  • HIST2H2BE
  • Gene Alias:
  • GL105,H2B,H2B.1,H2B/q,H2BFQ,MGC119802,MGC119804,MGC129733,MGC129734
  • Gene Description:
  • histone cluster 2, H2be
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
  • Other Designations:
  • H2B histone family, member Q,OTTHUMP00000013920,histone 2, H2be
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