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Histone H2A monoclonal antibody, clone RM225

  • Catalog # : MAB15118
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human histone H2A.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human histone H2A.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.2-1 ug/mL)
    Immunocytochemistry (1-2 ug/mL)
    Multiplex (0.2-1 ug/mL)
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: recombinant Histone H2A, Lane 2: recombinant Histone H2B, Lane 3: HeLa, Lane 4: A375, Lane 5: SK-MEL-2, Lane 6: A431, Lane 7: K562 whole cell lysates with Histone H2A monoclonal antibody, clone RM225 (Cat # MAB15118) at 0.5 ug/mL working concentration.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of HeLa cells with Histone H2A monoclonal antibody, clone RM225 (Cat # MAB15118) (Red). Actin filaments was labeled with fluorescein phalloidin (Green).
  • ELISA
  • Application Image
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3012
  • Protein Accession#:
  • P04908; Q93077; Q7L7L0; Q6FI13; P20671; P0C0S8
  • Gene Name:
  • HIST1H2AE
  • Gene Alias:
  • H2A.1,H2A.2,H2A/a,H2AFA
  • Gene Description:
  • histone cluster 1, H2ae
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
  • Other Designations:
  • H2A histone family, member A,histone 1, H2ae,histone H2AE
  • Gene Information
  • Entrez GeneID:
  • 8334
  • Protein Accession#:
  • P04908; Q93077; Q7L7L0; Q6FI13; P20671; P0C0S8
  • Gene Name:
  • HIST1H2AC
  • Gene Alias:
  • H2A/l,H2AFL,MGC99519,dJ221C16.4
  • Gene Description:
  • histone cluster 1, H2ac
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
  • Other Designations:
  • H2A histone family, member L,OTTHUMP00000016145,histone 1, H2ac,histone H2AC
  • Gene Information
  • Protein Accession#:
  • P04908; Q93077; Q7L7L0; Q6FI13; P20671; P0C0S8
  • Gene Name:
  • HIST2H2AA4
  • Gene Alias:
  • H2A/R
  • Gene Description:
  • histone cluster 2, H2aa4
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000013922,histone 2, H2aa4,histone H2A/r
  • Gene Information
  • Entrez GeneID:
  • 3013
  • Protein Accession#:
  • P04908; Q93077; Q7L7L0; Q6FI13; P20671; P0C0S8
  • Gene Name:
  • HIST1H2AD
  • Gene Alias:
  • H2A.3,H2A/g,H2AFG,HIST1H3D
  • Gene Description:
  • histone cluster 1, H2ad
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
  • Other Designations:
  • H2A histone family, member G,OTTHUMP00000016153,histone 1, H2ad,histone H2AD
  • Gene Information
  • Entrez GeneID:
  • 8329
  • Protein Accession#:
  • P04908; Q93077; Q7L7L0; Q6FI13; P20671; P0C0S8
  • Gene Name:
  • HIST1H2AI
  • Gene Alias:
  • FLJ92027,H2A/c,H2AFC
  • Gene Description:
  • histone cluster 1, H2ai
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
  • Other Designations:
  • H2A histone family, member C,OTTHUMP00000016183,histone 1, H2ai
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