Histone H2A monoclonal antibody, clone RM225
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: recombinant Histone H2A, Lane 2: recombinant Histone H2B, Lane 3: HeLa, Lane 4: A375, Lane 5: SK-MEL-2, Lane 6: A431, Lane 7: K562 whole cell lysates with Histone H2A monoclonal antibody, clone RM225 (Cat # MAB15118) at 0.5 ug/mL working concentration.
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with Histone H2A monoclonal antibody, clone RM225 (Cat # MAB15118) (Red). Actin filaments was labeled with fluorescein phalloidin (Green).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human histone H2A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to C-terminus of human Histone H2A.
Sequence
N/A
Reactivity
Human
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.2-1 ug/mL)
Immunocytochemistry (1-2 ug/mL)
Multiplex (0.2-1 ug/mL)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: recombinant Histone H2A, Lane 2: recombinant Histone H2B, Lane 3: HeLa, Lane 4: A375, Lane 5: SK-MEL-2, Lane 6: A431, Lane 7: K562 whole cell lysates with Histone H2A monoclonal antibody, clone RM225 (Cat # MAB15118) at 0.5 ug/mL working concentration.Immunocytochemistry
Immunocytochemistry staining of HeLa cells with Histone H2A monoclonal antibody, clone RM225 (Cat # MAB15118) (Red). Actin filaments was labeled with fluorescein phalloidin (Green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HIST1H2AE
Entrez GeneID
3012Protein Accession#
P04908; Q93077; Q7L7L0; Q6FI13; P20671; P0C0S8Gene Name
HIST1H2AE
Gene Alias
H2A.1, H2A.2, H2A/a, H2AFA
Gene Description
histone cluster 1, H2ae
Omim ID
602786Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H2A histone family, member A|histone 1, H2ae|histone H2AE
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Gene Info — HIST1H2AD
Entrez GeneID
3013Protein Accession#
P04908; Q93077; Q7L7L0; Q6FI13; P20671; P0C0S8Gene Name
HIST1H2AD
Gene Alias
H2A.3, H2A/g, H2AFG, HIST1H3D
Gene Description
histone cluster 1, H2ad
Omim ID
602792Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H2A histone family, member G|OTTHUMP00000016153|histone 1, H2ad|histone H2AD
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Gene Info — HIST1H2AI
Entrez GeneID
8329Protein Accession#
P04908; Q93077; Q7L7L0; Q6FI13; P20671; P0C0S8Gene Name
HIST1H2AI
Gene Alias
FLJ92027, H2A/c, H2AFC
Gene Description
histone cluster 1, H2ai
Omim ID
602787Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H2A histone family, member C|OTTHUMP00000016183|histone 1, H2ai
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Gene Info — HIST1H2AC
Entrez GeneID
8334Protein Accession#
P04908; Q93077; Q7L7L0; Q6FI13; P20671; P0C0S8Gene Name
HIST1H2AC
Gene Alias
H2A/l, H2AFL, MGC99519, dJ221C16.4
Gene Description
histone cluster 1, H2ac
Omim ID
602794Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H2A histone family, member L|OTTHUMP00000016145|histone 1, H2ac|histone H2AC
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Gene Info — HIST2H2AA4
Entrez GeneID
723790Protein Accession#
P04908; Q93077; Q7L7L0; Q6FI13; P20671; P0C0S8Gene Name
HIST2H2AA4
Gene Alias
H2A/R
Gene Description
histone cluster 2, H2aa4
Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq
Other Designations
OTTHUMP00000013922|histone 2, H2aa4|histone H2A/r
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