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5-methylcytosine (5-mC) monoclonal antibody, clone RM231

  • Catalog # : MAB15115
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against 5-methylcytosine (5-mC).
  • Immunogen:
  • 5-methylcytosine conjugated with BSA.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Dot Blot (0.5-2 ug/mL)
    ELISA (0.1-1 ug/mL)
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Methylated DNA Immunoprecipitation (0.2-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of HeLa cells with 5-methylcytosine (5-mC) monoclonal antibody, clone RM231 (Cat # MAB15115) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green), and nuclei stained with DAPI (Blue).
  • ELISA
  • ELISA
  • ELISA analysis of single stranded DNA with 5-methylcytosine (5-mC) monoclonal antibody, clone RM231 (Cat # MAB15115). The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of MAB15115 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
  • Dot Blot
  • Dot Blot
  • Dot blot analysis of double stranded DNA with 5-methylcytosine (5-mC) monoclonal antibody, clone RM231 (Cat # MAB15115). The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with MAB15115.
  • Methylated DNA Immunoprecipitation
  • Methylated DNA Immunoprecipitation
  • Methylated DNA Immunoprecipitation (MeDIP) analysis of 5-methylcytosine (5-mC) monoclonal antibody, clone RM231 (Cat # MAB15115) at a 2:1 DNA:Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1 ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Methylated DNA Immunoprecipitation
  • Methylated DNA Immunoprecipitation
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