Histone H2B (phospho S14) monoclonal antibody, clone RM238
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: acid extracts of HeLa cell treated with Nocodazole and Lane 2: acid extracts of HeLa cell with Histone H2B (phospho S14) monoclonal antibody, clone RM238 (Cat # MAB15114) at 0.5 ug/mL working concentration.
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with Histone H2B (phospho S14) monoclonal antibody, clone RM238 (Cat # MAB15114) (Red) and DAPI (Blue). Actin filaments have been labeled with fluorescein phalloidin (Green).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human histone H2B (S14).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S14 of human Histone H2B.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to histone H2B only when phosphorylated at S14. No cross reactivity with other phosphorylated histones.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.2-1 ug/mL)
Immunocytochemistry (1-2 ug/mL)
Multiplex (0.1-1 ug/mL)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: acid extracts of HeLa cell treated with Nocodazole and Lane 2: acid extracts of HeLa cell with Histone H2B (phospho S14) monoclonal antibody, clone RM238 (Cat # MAB15114) at 0.5 ug/mL working concentration.Immunocytochemistry
Immunocytochemistry staining of HeLa cells with Histone H2B (phospho S14) monoclonal antibody, clone RM238 (Cat # MAB15114) (Red) and DAPI (Blue). Actin filaments have been labeled with fluorescein phalloidin (Green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HIST1H2BB
Entrez GeneID
3018Protein Accession#
P33778Gene Name
HIST1H2BB
Gene Alias
H2B.1, H2B/f, H2BFF, MGC119804
Gene Description
histone cluster 1, H2bb
Omim ID
602803Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H2B histone family, member F|OTTHUMP00000016138|histone 1, H2bb
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