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Histone H2B (acetyl K20) monoclonal antibody, clone RM235

  • Catalog # : MAB15112
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic acetyl peptide of human histone H2B (acetyl K20).
  • Immunogen:
  • A synthetic acetyl peptide corresponding to residues surrounding K20 of human histone H2B.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to histone H2B acetylated at K20. No cross reactivity with non-modified K20 or other acetylated lysines in histone H2B.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.2-1 ug/mL)
    Immunocytochemistry (1-2 ug/mL)
    Multiplex (0.1-0.5 ug/mL)
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: acid extracts of HeLa cell treated with sodium butyrate and Lane 2: acid extracts of HeLa cell with Histone H2B (acetyl K20) monoclonal antibody, clone RM235 (Cat # MAB15112) at 0.5 ug/mL working concentration.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of HeLa cells treated with sodium butyrate using Histone H2B (acetyl K20) monoclonal antibody, clone RM235 (Cat # MAB15112) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3018
  • Gene Name:
  • Gene Alias:
  • H2B.1,H2B/f,H2BFF,MGC119804
  • Gene Description:
  • histone cluster 1, H2bb
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
  • Other Designations:
  • H2B histone family, member F,OTTHUMP00000016138,histone 1, H2bb
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