Histone H2AX (phospho S139) monoclonal antibody, clone RM224
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: acid extracts of HeLa cell treated with UV and Lane 2: acid extracts of HeLa cell with Histone H2AX (phospho S139) monoclonal antibody, clone RM224 (Cat # MAB15111) at 0.5 ug/mL working concentration.
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with Histone H2AX (phospho S139) monoclonal antibody, clone RM224 (Cat # MAB15111) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human histone H2AX (S139).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S139 of human Histone H2AX.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to histone H2AX only when phosphorylated at S139. No cross reactivity with other phosphorylated histones.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.2-1 ug/mL)
Immunocytochemistry (0.5-2 ug/mL)
Multiplex (0.1-1 ug/mL)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: acid extracts of HeLa cell treated with UV and Lane 2: acid extracts of HeLa cell with Histone H2AX (phospho S139) monoclonal antibody, clone RM224 (Cat # MAB15111) at 0.5 ug/mL working concentration.Immunocytochemistry
Immunocytochemistry staining of HeLa cells with Histone H2AX (phospho S139) monoclonal antibody, clone RM224 (Cat # MAB15111) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — H2AFX
Entrez GeneID
3014Protein Accession#
P16104Gene Name
H2AFX
Gene Alias
H2A.X, H2A/X, H2AX
Gene Description
H2A histone family, member X
Omim ID
601772Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
Other Designations
H2AX histone
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Interactome
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Pathway
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Disease
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