Western Blot analysis of Lane 1: acid extracts of HeLa cell treated with UV and Lane 2: acid extracts of HeLa cell with Histone H2AX (phospho S139) monoclonal antibody, clone RM224 (Cat # MAB15111) at 0.5 ug/mL working concentration.
Immunocytochemistry staining of HeLa cells with Histone H2AX (phospho S139) monoclonal antibody, clone RM224 (Cat # MAB15111) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq