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Histone H2AX (phospho S139) monoclonal antibody, clone RM224

  • Catalog # : MAB15111
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic phosphopeptide of human histone H2AX (S139).
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S139 of human histone H2AX.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to histone H2AX only when phosphorylated at S139. No cross reactivity with other phosphorylated histones.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.2-1 ug/mL)
    Immunocytochemistry (0.5-2 ug/mL)
    Multiplex (0.1-1 ug/mL)
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: acid extracts of HeLa cell treated with UV and Lane 2: acid extracts of HeLa cell with Histone H2AX (phospho S139) monoclonal antibody, clone RM224 (Cat # MAB15111) at 0.5 ug/mL working concentration.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of HeLa cells with Histone H2AX (phospho S139) monoclonal antibody, clone RM224 (Cat # MAB15111) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3014
  • Gene Name:
  • H2AFX
  • Gene Alias:
  • H2A.X,H2A/X,H2AX
  • Gene Description:
  • H2A histone family, member X
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
  • Other Designations:
  • H2AX histone
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