Mouse IgG2c monoclonal antibody, clone RM223
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2c with Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15110) under 0.5 ug/mL working concentration. This antibody reacts to nonreduced IgG2c.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15110) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of mouse IgG2c. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA of IgG from BalB/C and C57BL6 shows Mouse IgG2c monoclonal antibody, clone RM223 (Cat# MAB15110) reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of Cat# MAB15110 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse IgG2c.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse IgG2c.
Sequence
N/A
Reactivity
Mouse
Specificity
This antibody reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.01-0.2 ug/mL)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-2 ug/mL)
Immunoprecipitation
Western Blot (nonreduced only) (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2c with Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15110) under 0.5 ug/mL working concentration. This antibody reacts to nonreduced IgG2c.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15110) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of mouse IgG2c. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
ELISA of IgG from BalB/C and C57BL6 shows Mouse IgG2c monoclonal antibody, clone RM223 (Cat# MAB15110) reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of Cat# MAB15110 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — Igh-1b
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