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Last updated: 2017/4/23

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Mouse IgG2c monoclonal antibody, clone RM223

  • Catalog # : MAB15110
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against native mouse IgG2c.
  • Immunogen:
  • Native purified IgG2c from mouse hybridoma.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Specificity:
  • This antibody reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.01-0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-2 ug/mL)
    Immunoprecipitation
    Western Blot (nonreduced only) (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2c with Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15110) under 0.5 ug/mL working concentration. This antibody reacts to nonreduced IgG2c.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • ELISA
  • ELISA analysis of Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15110) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of mouse IgG2c. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • Igh-1b
  • Gene Alias:
  • IGHG2C
  • Gene Description:
  • immunoglobulin heavy chain 1b (serum IgG2c)
  • Other Designations:
  • -
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