CD22 monoclonal antibody, clone BLCAM/1795
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Raji and Lane 2: Ramos cell lysates with CD22 monoclonal antibody, clone BLCAM/1795 (Cat # MAB15057).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with CD22 monoclonal antibody, clone BLCAM/1795 (Cat # MAB15057).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CD22.
Immunogen
Recombinant protein corresponding to amino acids 52-178 of human CD22.
Host
Mouse
Theoretical MW (kDa)
130-140
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA (2-4 ug/mL for coating)
Flow Cytometry (0.5-1 ug/106 cells)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
Western Blotting (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Raji and Lane 2: Ramos cell lysates with CD22 monoclonal antibody, clone BLCAM/1795 (Cat # MAB15057).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with CD22 monoclonal antibody, clone BLCAM/1795 (Cat # MAB15057).Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — CD22
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Interactome
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Pathway
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Disease
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Publication Reference
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CD22 negatively and positively regulates signal transduction through the B lymphocyte antigen receptor.
Sato S, Tuscano JM, Inaoki M, Tedder TF.
Seminars in Immunology 1998 Aug; 10(4):287.
Application：Flow Cyt, Human, Mouse, B lymphocytes.
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CD22 negatively and positively regulates signal transduction through the B lymphocyte antigen receptor.
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