Villin monoclonal antibody, clone VIL1/1325
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: A549, Lane 2: HepG2, and Lane 3: HCT116 cell lysates with Villin monoclonal antibody, clone VIL1/1325 (Cat # MAB15030).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rectum (A) and human colon (B) with Villin monoclonal antibody, clone VIL1/1325 (Cat # MAB15030).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human Villin.
Immunogen
Recombinant protein corresponding to 133 residues of human Villin.
Sequence
MGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGA
Host
Mouse
Theoretical MW (kDa)
93
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
Western Blotting (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: A549, Lane 2: HepG2, and Lane 3: HCT116 cell lysates with Villin monoclonal antibody, clone VIL1/1325 (Cat # MAB15030).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rectum (A) and human colon (B) with Villin monoclonal antibody, clone VIL1/1325 (Cat # MAB15030).Immunofluorescence
Flow Cytometry
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Publication Reference
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Distribution and pattern of expression of villin, a gastrointestinal-associated cytoskeletal protein, in human carcinomas: a study employing paraffin-embedded tissue.
Bacchi CE, Gown AM.
Laboratory Investigation 1991 Mar; 64(3):418.
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Localization of villin, a cytoskeletal protein specific to microvilli, in human ileum and colon and in colonic neoplasms.
West AB, Isaac CA, Carboni JM, Morrow JS, Mooseker MS, Barwick KW.
Gastroenterology 1988 Feb; 94(2):343.
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Distribution and pattern of expression of villin, a gastrointestinal-associated cytoskeletal protein, in human carcinomas: a study employing paraffin-embedded tissue.
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