S100B monoclonal antibody, clone 4C4.9 + S100B/1012
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human melanoma with S100B monoclonal antibody, clone 4C4.9 + S100B/1012 (Cat # MAB14983).
Immunofluorescence
Immunofluorescent staining of A2058 cells with S100B monoclonal antibody, clone 4C4.9 + S100B/1012 (Cat # MAB14983) (Green). Blue: DAPI was used to stain the cell nuclei. Red: F-actin filaments were labeled with DyLight 554 Phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against S100B.
Immunogen
Native purified S100 from bovine brain and recombinant protein corresponding to full length human S100B.
Host
Mouse
Theoretical MW (kDa)
10-12
Reactivity
Bovine, Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
Western Blotting (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human melanoma with S100B monoclonal antibody, clone 4C4.9 + S100B/1012 (Cat # MAB14983).Immunofluorescence
Immunofluorescent staining of A2058 cells with S100B monoclonal antibody, clone 4C4.9 + S100B/1012 (Cat # MAB14983) (Green). Blue: DAPI was used to stain the cell nuclei. Red: F-actin filaments were labeled with DyLight 554 Phalloidin.Flow Cytometry
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Gene Info — S100B
Entrez GeneID
6285Protein Accession#
P02638;P04271Gene Name
S100B
Gene Alias
NEF, S100, S100beta
Gene Description
S100 calcium binding protein B
Omim ID
176990Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq
Other Designations
OTTHUMP00000174958|S-100 calcium-binding protein, beta chain|S100 beta|S100 calcium binding protein, beta (neural)|S100 calcium-binding protein, beta|S100 calcium-binding protein, beta (neural)
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Gene Info — S100B
Entrez GeneID
525716Protein Accession#
P02638;P04271Gene Name
S100B
Gene Alias
MGC128275
Gene Description
S100 calcium binding protein B
Gene Ontology
HyperlinkGene Summary
beta polypeptide
Other Designations
S100 protein, beta polypeptide
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Interactome
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Disease
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Publication Reference
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The immunohistochemical localization of S100 in the diagnosis of papillary carcinoma of the thyroid.
McLaren KM, Cossar DW.
Human Pathology 1996 Jul; 27(7):633.
Application：IHC-P, Human, Human papillary carcinoma of the thyroid.
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The role of immunocytochemical markers in the differential diagnosis of proliferative and neoplastic lesions of the breast.
Joshi MG, Lee AK, Pedersen CA, Schnitt S, Camus MG, Hughes KS.
Modern Pathology 1996 Jan; 9(1):57.
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The immunohistochemical localization of S100 in the diagnosis of papillary carcinoma of the thyroid.
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