S100B monoclonal antibody, clone S100B/1012
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human melanoma (A) and human schwannoma (B) with S100B monoclonal antibody, clone S100B/1012 (Cat # MAB14981).
Immunofluorescence
Immunofluorescent staining of A2058 cells with S100B monoclonal antibody, clone S100B/1012 (Cat # MAB14981) (Green). Blue: DAPI was used to stain the cell nuclei. Red: F-actin filaments were labeled with DyLight 554 Phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human S100B.
Immunogen
Recombinant protein corresponding to full length human S100B.
Host
Mouse
Theoretical MW (kDa)
10-12
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
Western Blotting (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human melanoma (A) and human schwannoma (B) with S100B monoclonal antibody, clone S100B/1012 (Cat # MAB14981).Immunofluorescence
Immunofluorescent staining of A2058 cells with S100B monoclonal antibody, clone S100B/1012 (Cat # MAB14981) (Green). Blue: DAPI was used to stain the cell nuclei. Red: F-actin filaments were labeled with DyLight 554 Phalloidin.Flow Cytometry
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Gene Info — S100B
Entrez GeneID
6285Protein Accession#
P04271Gene Name
S100B
Gene Alias
NEF, S100, S100beta
Gene Description
S100 calcium binding protein B
Omim ID
176990Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq
Other Designations
OTTHUMP00000174958|S-100 calcium-binding protein, beta chain|S100 beta|S100 calcium binding protein, beta (neural)|S100 calcium-binding protein, beta|S100 calcium-binding protein, beta (neural)
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Interactome
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Disease
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Publication Reference
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Calcium-binding proteins of the EF-type.
Heizmann CW.
Journal of Cardiovascular Pharmacology 1988 May; 12 Suppl 5:S30.
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Calcium-binding proteins of the EF-type.
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