His-Tag monoclonal antibody, clone RM146 (Biotin)
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of 293T cell lysate Lane 1: transfected and Lane 2: untransfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein using His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964) at 0.2 ug/mL working concentration, followed by a HRP conjugated Streptavidin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964) at the following concentrations: 0.5 ug/mL, 0.25 ug/mL, 0.125 ug/mL, 0.063 ug/mL, 0.031 ug/mL, 0.016 ug/mL, 0.008 ug/mL. The plate performed on purified HisTag-PCSK9 recombinant protein. An alkaline phosphatase conjugated streptavidin as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against His-Tag.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a mixture of synthetic peptides corresponding to N-terminus region of 6X His-Tag and C-terminus region of 6X His-Tag.
Sequence
N/A
Form
Liquid
Conjugation
Biotin
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.01-0.5 ug/mL)
Flow Cytometry (0.5-2 ug/mL)
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-1 ug/mL)
Immunoprecipitation
Western Blotting (0.1-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of 293T cell lysate Lane 1: transfected and Lane 2: untransfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein using His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964) at 0.2 ug/mL working concentration, followed by a HRP conjugated Streptavidin.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964).Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964) at the following concentrations: 0.5 ug/mL, 0.25 ug/mL, 0.125 ug/mL, 0.063 ug/mL, 0.031 ug/mL, 0.016 ug/mL, 0.008 ug/mL. The plate performed on purified HisTag-PCSK9 recombinant protein. An alkaline phosphatase conjugated streptavidin as the secondary antibody.Flow Cytometry
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