Rabbit monoclonal antibody raised against His-Tag.
A mixture of synthetic peptides corresponding to N-terminus region of 6X His-Tag and C-terminus region of 6X His-Tag.
Protein A purification
ELISA (0.01-0.5 ug/mL) Flow Cytometry (0.5-2 ug/mL) Immunocytochemistry (0.5-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-1 ug/mL) Immunoprecipitation Western Blotting (0.1-0.5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of 293T cell lysate Lane 1: transfected and Lane 2: untransfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein using His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964) at 0.2 ug/mL working concentration, followed by a HRP conjugated Streptavidin.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964).
ELISA analysis of His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964) at the following concentrations: 0.5 ug/mL, 0.25 ug/mL, 0.125 ug/mL, 0.063 ug/mL, 0.031 ug/mL, 0.016 ug/mL, 0.008 ug/mL. The plate performed on purified HisTag-PCSK9 recombinant protein. An alkaline phosphatase conjugated streptavidin as the secondary antibody.