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His-Tag monoclonal antibody, clone RM146 (Biotin)

  • Catalog # : MAB14964
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against His-Tag.
  • Immunogen:
  • A mixture of synthetic peptides corresponding to N-terminus region of 6X His-Tag and C-terminus region of 6X His-Tag.
  • Host:
  • Rabbit
  • Form:
  • Liquid
  • Conjugation:
  • Biotin
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.01-0.5 ug/mL)
    Flow Cytometry (0.5-2 ug/mL)
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-1 ug/mL)
    Immunoprecipitation
    Western Blotting (0.1-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of 293T cell lysate Lane 1: transfected and Lane 2: untransfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein using His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964) at 0.2 ug/mL working concentration, followed by a HRP conjugated Streptavidin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964).
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • ELISA
  • ELISA analysis of His-Tag monoclonal antibody, clone RM146 (Biotin) (Cat # MAB14964) at the following concentrations: 0.5 ug/mL, 0.25 ug/mL, 0.125 ug/mL, 0.063 ug/mL, 0.031 ug/mL, 0.016 ug/mL, 0.008 ug/mL. The plate performed on purified HisTag-PCSK9 recombinant protein. An alkaline phosphatase conjugated streptavidin as the secondary antibody.
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunoprecipitation
  • Flow Cytometry
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