ACTB monoclonal antibody, clone RM112 (Biotin)
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A431 cell lysate with ACTB monoclonal antibody, clone RM112 (Biotin) (Cat # MAB14962) at 1:1000 dilution.
Immunohistochemistry
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue using ACTB monoclonal antibody, clone RM112 (Biotin) (Cat# MAB14962) at a 1:1000 dilution.
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with ACTB monoclonal antibody, clone RM112 (Biotin) (Cat # MAB14962) (Red) at 1:200 dilution. Blue: DAPI was used to stain the cell nuclei.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of ACTB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to N-terminus of human ACTB.
Sequence
N/A
Form
Liquid
Conjugation
Biotin
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ChIP (1:200)
ELISA
Flow Cytometry
Immunocytochemistry (1:200)
Immunohistochemistry (1:200)
Immunoprecipitation (1:200)
Western Blotting (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Cell lysate)
Western blot analysis of A431 cell lysate with ACTB monoclonal antibody, clone RM112 (Biotin) (Cat # MAB14962) at 1:1000 dilution.Immunohistochemistry
Immunohistochemistry
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue using ACTB monoclonal antibody, clone RM112 (Biotin) (Cat# MAB14962) at a 1:1000 dilution.Immunocytochemistry
Immunocytochemistry staining of HeLa cells with ACTB monoclonal antibody, clone RM112 (Biotin) (Cat # MAB14962) (Red) at 1:200 dilution. Blue: DAPI was used to stain the cell nuclei.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — ACTB
Entrez GeneID
60Protein Accession#
P60709Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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Interactome
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Pathway
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Disease
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