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Last updated: 2017/3/26

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Human IgG4 monoclonal antibody, clone RM120 (Biotin)

  • Catalog # : MAB14959
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human IgG4.
  • Immunogen:
  • A synthetic peptide corresponding to the hinge region of human IgG4.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Conjugation:
  • Biotin
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.05-0.2 ug/well for capture, 0.05-0.2 ug/mL for detection)
    Flow Cytometry
    Immunocytochemistry (1-10 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-10 ug/mL)
    Western Blotting (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of human, mouse, rat, and goat IgG with Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat # MAB14959) at 0.5 ug/mL working concentration.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat # MAB14959).
  • Immunocytochemistry
  • ELISA
  • ELISA
  • ELISA analysis of Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 3503
  • Gene Name:
  • IGHG4
  • Gene Alias:
  • MGC117419
  • Gene Description:
  • immunoglobulin heavy constant gamma 4 (G4m marker)
  • Other Designations:
  • -
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