Rabbit monoclonal antibody raised against synthetic peptide of human IgG4.
A synthetic peptide corresponding to the hinge region of human IgG4.
Protein A purification
ELISA (0.05-0.2 ug/well for capture, 0.05-0.2 ug/mL for detection) Flow Cytometry Immunocytochemistry (1-10 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-10 ug/mL) Western Blotting (0.5-2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat # MAB14959).
ELISA analysis of Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.