Human IgG4 monoclonal antibody, clone RM120 (Biotin)
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More Files
- More Functions
Western Blot
Western blot analysis of human, mouse, rat, and goat IgG with Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat # MAB14959) at 0.5 ug/mL working concentration.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat # MAB14959).
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat# MAB14959) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat# MAB14959) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human IgG4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to the hinge region of human IgG4.
Sequence
N/A
Reactivity
Human
Form
Liquid
Conjugation
Biotin
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.05-0.2 ug/well for capture, 0.05-0.2 ug/mL for detection)
Flow Cytometry
Immunocytochemistry (1-10 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-10 ug/mL)
Western Blotting (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of human, mouse, rat, and goat IgG with Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat # MAB14959) at 0.5 ug/mL working concentration.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat # MAB14959).Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat# MAB14959) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG4 monoclonal antibody, clone RM120 (Biotin) (Cat# MAB14959) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — IGHG4
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Interactome
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