Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Biotin)
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Biotin) (Cat # MAB14948).
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Biotin) (Cat# MAB14948) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Cat # MAB12808) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the gamma 1, gamma 2, gamma3, gamma 4 of human IgGs and the Fc of human IgG; no cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Biotin) (Cat# MAB14948) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA using RM116. The plate was coated with different amounts of human IgG1. A serial dilution of Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Biotin) (Cat# MAB14948) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IgG (gamma heavy chain).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse IgG.
Sequence
N/A
Reactivity
Human
Form
Liquid
Conjugation
Biotin
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.05-0.2 ug/well for capture, 0.05-0.2 ug/mL for detection)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Biotin) (Cat # MAB14948).Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Biotin) (Cat# MAB14948) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Cat # MAB12808) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the gamma 1, gamma 2, gamma3, gamma 4 of human IgGs and the Fc of human IgG; no cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Biotin) (Cat# MAB14948) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.Enzyme-linked Immunoabsorbent Assay
A titer ELISA using RM116. The plate was coated with different amounts of human IgG1. A serial dilution of Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Biotin) (Cat# MAB14948) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — IGHG1
Entrez GeneID
3500Protein Accession#
P01857;P01859;P01860;P01861Gene Name
IGHG1
Gene Alias
-
Gene Description
immunoglobulin heavy constant gamma 1 (G1m marker)
Omim ID
147100Gene Ontology
HyperlinkOther Designations
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Gene Info — IGHG2
Entrez GeneID
3501Protein Accession#
P01857;P01859;P01860;P01861Gene Name
IGHG2
Gene Alias
DKFZp686I04196
Gene Description
immunoglobulin heavy constant gamma 2 (G2m marker)
Omim ID
147110Gene Ontology
HyperlinkOther Designations
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Gene Info — IGHG3
Entrez GeneID
3502Protein Accession#
P01857;P01859;P01860;P01861Gene Name
IGHG3
Gene Alias
DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, MGC45809
Gene Description
immunoglobulin heavy constant gamma 3 (G3m marker)
Omim ID
147120Gene Ontology
HyperlinkOther Designations
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Gene Info — IGHG4
Entrez GeneID
3503Protein Accession#
P01857;P01859;P01860;P01861Gene Name
IGHG4
Gene Alias
MGC117419
Gene Description
immunoglobulin heavy constant gamma 4 (G4m marker)
Omim ID
147130Gene Ontology
HyperlinkOther Designations
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Interactome
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Disease
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