Rabbit IgG Fab monoclonal antibody, clone RMG01 (Biotin)
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) rabbit IgG (20 ng/lane) with Rabbit IgG Fab monoclonal antibody, clone RMG01 (Biotin) (Cat # MAB14943) at 0.2 ug/mL working concentration. This antibody reacted to nonreduced rabbit IgG (~150 kDa).
Enzyme-linked Immunoabsorbent Assay
A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of Rabbit IgG Fab monoclonal antibody, clone RMG01 (Biotin) (Cat# MAB14943) was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IgG from different species with Rabbit IgG Fab monoclonal antibody, clone RMG01 (Cat # MAB12773) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different IgG and the result shows this antibody reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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Specification
Product Description
Goat recombinant monoclonal antibody raised against rabbit IgG Fab.
Antibody Species
Goat
Immunogen
Original antibody is raised against rabbit IgG.
Sequence
N/A
Reactivity
Rabbit
Form
Liquid
Conjugation
Biotin
Purification
Protein G purification
Isotype
IgG
Recommend Usage
ELISA (0.01-0.5 ug/mL)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
Immunoprecipitation
Western Blotting (0.1-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) rabbit IgG (20 ng/lane) with Rabbit IgG Fab monoclonal antibody, clone RMG01 (Biotin) (Cat # MAB14943) at 0.2 ug/mL working concentration. This antibody reacted to nonreduced rabbit IgG (~150 kDa).Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of Rabbit IgG Fab monoclonal antibody, clone RMG01 (Biotin) (Cat# MAB14943) was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IgG from different species with Rabbit IgG Fab monoclonal antibody, clone RMG01 (Cat # MAB12773) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different IgG and the result shows this antibody reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.Flow Cytometry
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