Rabbit monoclonal antibody raised against native mouse IgA.
Native purified mouse IgA.
Protein A purification
ELISA (0.005-0.2 ug/mL) Flow Cytometry Immunocytochemistry (0.5-2 ug/mL) Immunohistochemistry (0.5-2 ug/mL) Immunoprecipitation Western Blotting (0.5-2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgA with Mouse IgA monoclonal antibody, clone RM220 (Biotin) (Cat # MAB14940) at 0.5 ug/mL working concentration. This antibody reacts to nonreduced IgA.
ELISA analysis of Mouse IgA monoclonal antibody, clone RM220 (Cat # MAB12771) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.