Rabbit monoclonal antibody raised against native mouse IgG2b.
Native purified mouse IgG2b.
Protein A purification
ELISA (0.005-0.2 ug/mL) Flow Cytometry Immunocytochemistry (0.5-2 ug/mL) Immunohistochemistry (0.5-2 ug/mL) Immunoprecipitation Western Blotting (0.1-0.5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2b (20 ng/lane) with Mouse IgG2b monoclonal antibody, clone RM108 (Biotin) (Cat # MAB14938) at 0.2 ug/mL working concentration. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced gamma 2b form (~50 kDa).
ELISA analysis of Mouse IgG2b monoclonal antibody, clone RM108 (Cat # MAB12769) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.