Mouse IgG2a monoclonal antibody, clone RM219 (Biotin)
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2a with Mouse IgG2a monoclonal antibody, clone RM219 (Biotin) (Cat # MAB14937) at 0.5 ug/mL working concentration. This antibody reacts to nonreduced IgG2a (~150 kDa).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG2a monoclonal antibody, clone RM219 (Cat # MAB12768) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of Mouse IgG2a monoclonal antibody, clone RM219 (Biotin) (Cat# MAB14937) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse IgG2a.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse IgG2a.
Sequence
N/A
Reactivity
Mouse
Form
Liquid
Conjugation
Biotin
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.005-0.2 ug/mL)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
Immunoprecipitation
Western Blotting (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2a with Mouse IgG2a monoclonal antibody, clone RM219 (Biotin) (Cat # MAB14937) at 0.5 ug/mL working concentration. This antibody reacts to nonreduced IgG2a (~150 kDa).Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG2a monoclonal antibody, clone RM219 (Cat # MAB12768) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of Mouse IgG2a monoclonal antibody, clone RM219 (Biotin) (Cat# MAB14937) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — Igh-1a
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