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Last updated: 2017/3/26

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Mouse IgG monoclonal antibody, clone RM104 (Biotin)

  • Catalog # : MAB14934
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against native mouse IgG.
  • Immunogen:
  • Native purified mouse IgG.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Form:
  • Liquid
  • Conjugation:
  • Biotin
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.005-0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    Immunoprecipitation
    Western Blotting (0.1-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of nonreduced (-) and reduced (+) mouse immunoglobulins (20 ng/lane) with Mouse IgG monoclonal antibody, clone RM104 (Biotin) (Cat # MAB14934) at 0.2 ug/mL working concentration. This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • ELISA
  • ELISA analysis of Mouse IgG monoclonal antibody, clone RM104 (Cat # MAB12765) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • Ighg1
  • Gene Alias:
  • IgG1,Igh-4,VH7183
  • Gene Description:
  • immunoglobulin heavy constant gamma 1 (G1m marker)
  • Other Designations:
  • -
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