ELISA (0.005-0.2 ug/mL) Flow Cytometry Immunocytochemistry (0.5-2 ug/mL) Immunohistochemistry (0.5-2 ug/mL) Immunoprecipitation Western Blotting (0.1-0.5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of nonreduced (-) and reduced (+) mouse IgG1 (kappa) and IgG1 (lambda) (20 ng/lane) with Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103 (Biotin) (Cat # MAB14933) at 0.2 ug/mL working concentration. This antibody reacts to nonreduced IgG1 (kappa) (~150 kDa), and slightly reacts to reduced kappa light chain (~25 kDa).
ELISA analysis of Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103 (Cat # MAB12764) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.