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Last updated: 2017/3/26

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Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103 (Biotin)

  • Catalog # : MAB14933
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against native mouse immunoglobulin kappa light chain.
  • Immunogen:
  • Native purified mouse IgG.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Form:
  • Liquid
  • Conjugation:
  • Biotin
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.005-0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    Immunoprecipitation
    Western Blotting (0.1-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of nonreduced (-) and reduced (+) mouse IgG1 (kappa) and IgG1 (lambda) (20 ng/lane) with Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103 (Biotin) (Cat # MAB14933) at 0.2 ug/mL working concentration. This antibody reacts to nonreduced IgG1 (kappa) (~150 kDa), and slightly reacts to reduced kappa light chain (~25 kDa).
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • ELISA
  • ELISA analysis of Mouse immunoglobulin kappa light chain monoclonal antibody, clone RM103 (Cat # MAB12764) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • Igk-C
  • Gene Alias:
  • IGKC,MGC118128
  • Gene Description:
  • immunoglobulin kappa chain, constant region
  • Other Designations:
  • -
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