NEFL monoclonal antibody, clone RM280
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More Files
- More Functions
Western Blot (Tissue lysate)
Western Blot analysis of human brain tissue lysate with NEFL monoclonal antibody, clone RM280 (Cat # MAB14927).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain with NEFL monoclonal antibody, clone RM280 (Cat # MAB14927).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human NEFL.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to C-terminus of human NEFL.
Sequence
N/A
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:2500)
Western Blotting (1:1000-1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot analysis of human brain tissue lysate with NEFL monoclonal antibody, clone RM280 (Cat # MAB14927).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain with NEFL monoclonal antibody, clone RM280 (Cat # MAB14927). -
Gene Info — NEFL
Entrez GeneID
4747Protein Accession#
P07196Gene Name
NEFL
Gene Alias
CMT1F, CMT2E, FLJ53642, NF-L, NF68, NFL
Gene Description
neurofilament, light polypeptide
Gene Ontology
HyperlinkGene Summary
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq
Other Designations
light molecular weight neurofilament protein|neurofilament protein, light chain|neurofilament subunit NF-L|neurofilament triplet L protein|neurofilament, light polypeptide 68kDa|neurofilament-light
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Interactome
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Pathway
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Disease
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