DIABLO monoclonal antibody, clone RM271
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of HeLa cell (1) and MCF-7 cell (2) with DIABLO monoclonal antibody, clone RM271 (Cat # MAB14915).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with DIABLO monoclonal antibody, clone RM271 (Cat # MAB14915).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human DIABLO.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to the C-terminus of human DIABLO (Diablo homolog, mitochondrial).
Sequence
N/A
Reactivity
Human
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of HeLa cell (1) and MCF-7 cell (2) with DIABLO monoclonal antibody, clone RM271 (Cat # MAB14915).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with DIABLO monoclonal antibody, clone RM271 (Cat # MAB14915). -
Gene Info — DIABLO
Entrez GeneID
56616Gene Name
DIABLO
Gene Alias
DIABLO-S, FLJ10537, FLJ25049, SMAC, SMAC3
Gene Description
diablo homolog (Drosophila)
Omim ID
605219Gene Ontology
HyperlinkGene Summary
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq
Other Designations
0610041G12Rik|diablo|direct IAP-binding protein with low pI|mitochondrial Smac protein|second mitochondria-derived activator of caspase
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Interactome
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Disease
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