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CASP3 monoclonal antibody, clone RM250

  • Catalog # : MAB14911
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human CASP3.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human Caspase-3 subunit p17.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • Recognizes human full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa).
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:2500)
    Western Blot (1:1000-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate) analysis of Jurkat cell with CASP3 monoclonal antibody, clone RM250 (Cat # MAB14911). Jurkat cells treated (+) or nontreated (-) with etoposide.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with CASP3 monoclonal antibody, clone RM250 (Cat # MAB14911).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 836
  • Gene Name:
  • CASP3
  • Gene Alias:
  • CPP32,CPP32B,SCA-1
  • Gene Description:
  • caspase 3, apoptosis-related cysteine peptidase
  • Gene Summary:
  • This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000165054,PARP cleavage protease,SREBP cleavage activity 1,Yama,apopain,caspase 3,caspase 3, apoptosis-related cysteine protease,cysteine protease CPP32,procaspase3
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